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sali hf  (New England Biolabs)


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    Structured Review

    New England Biolabs sali hf
    Sali Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sali-hf/SalI-HF/pm42789724-511-45-50
    Average 98 stars, based on 540 article reviews
    sali hf - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Labeling:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Positive Control:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Infection:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Cell Culture:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Injection:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Enzyme-linked Immunosorbent Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Confocal Microscopy:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    FACS:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Reporter Gene Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Sonication:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    DNA Sequencing:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Isolation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Gel Purification:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Mouse Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    FLAG-tag:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Western Blot:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Filtration:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Bradford Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Spectrophotometry:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Nested PCR:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Produced:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Sequencing:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Irradiation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    shRNA:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Knock-Out:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Transferring:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Standard Deviation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Microarray:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Southern Blot:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    cDNA Library Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Subcloning:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    SYBR Green Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    DNA Ligation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Selection:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Purification:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    GUS Gene Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Electroporation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Magnetic Beads:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Conjugation Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Sampling:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Synthesized:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Radial Immuno Diffusion:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Binding Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Stripping Membranes:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    DNA Methylation Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    MTT Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Capsules:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Real-time Polymerase Chain Reaction:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    CtB Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Cytometry:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    High Performance Liquid Chromatography:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Incubation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    RNA HS Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Plasmid Preparation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    In Situ Hybridization:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Methylation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Gel Extraction:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Membrane:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Immunostaining:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    DNA Purification:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Reporter Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Transformation Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Knock-In:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Recombinant:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Bacteria:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Protein Concentration:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Modification:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Immunocytochemistry:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Computed Tomography:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Nucleic Acid Electrophoresis:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Lambda DNA Preparation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Polymerase Cycling Assembly:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Cell Isolation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Multiplex Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Over Expression:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Derivative Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    CRISPR:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Lysis:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Comparison:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Migration:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    RNA Sequencing Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Extraction:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Marker:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Clonogenic Cell Survival Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Variant Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Virus:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Sample Prep:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Immunohistochemistry:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    DNA Array:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Competitive Binding Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Ethanol Precipitation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Mug Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Buffer Exchange:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Polymerase Chain Reaction:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Activation Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Chromatin Immunoprecipitation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Negative Control:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Software:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Amplification:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Titration:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Mutagenesis:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    RNA Expression:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Ligation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Agarose Gel Electrophoresis:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    SDS Page:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Immunodetection:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Quantitation Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Suction Filtration:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Protein Binding:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    DNA Extraction:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Inhibition:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Microscopy:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Nanopore Sequencing:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Gentle:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Construct:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Hybridization:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Blocking Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Transmission Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    TA Cloning:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    In Vivo:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Transduction:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Proliferation Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    RNA In Situ Hybridization:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Bicinchoninic Acid Protein Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Plaque Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Fluorescence:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Suspension:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Sterility:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Activity Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Cell Viability Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Molecular Cloning:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Amplified Luminescent Proximity Homogenous Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Molecular Weight:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Countercurrent Chromatography:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Introduce:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Avidin-Biotin Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Protease Inhibitor:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Saline:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Expressing:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Concentration Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Random Primed:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    DNA Labeling:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Multiple Displacement Amplification:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Clone Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Flow Cytometry:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Random Hexamer Labeling:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Functional Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Imaging:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    HiChIP:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Centrifugation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Transgenic Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Generated:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Recombinase Polymerase Amplification:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    In Vitro:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Periodic Counter-current Chromatography:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Homologous Recombination:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Colony Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Luciferase:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Electrophoresis:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    DC Protein Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Transfection:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Quantitative RT-PCR:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Immunoprecipitation:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    Staining:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with

    HAT Assay:

    Article Title: Manufacturing DNA in E. coli yields higher-fidelity DNA than in vitro enzymatic synthesis
    Article Snippet: the concentration of the PCR purification (obtained using a nanodrop spectrophotometer) multiplied by the elution volume. The in - vivo - and in - vitro -synthesized DNAs were digested with SacI and SalI restriction enzymes (NEB R3156, R3138), as was a pUC19 vector with ampicillin resistance. We used a commercial gel extraction kit (Thermo Scientific K0691) to isolate the sacB-cat fragment and ligated each insert:vector combination overnight with



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